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. 2019 Oct 15;38(21):e102361. doi: 10.15252/embj.2019102361

Figure 7. The p97–ATX3 complex fine‐tunes histone H1 ubiquitination after DSBs.

Figure 7

  1. Representative IF images showing the average intensity of endogenous RNF168 at UV‐A micro‐laser‐induced DNA damage tracks in U2OS cells. Scale bar: 10 μm.
  2. Graph represents the quantification of (A) (***P < 0.001; unpaired t‐test, n = 3, mean + SEM, more than 50 nuclei were analysed).
  3. Representative IF images showing the average intensity of endogenous 53BP1 at UV‐A micro‐laser‐induced DNA damage tracks in U2OS cells. Scale bar: 10 μm.
  4. Graph represents the quantification of (C) (ns P > 0.05, ***P < 0.001; unpaired t‐test, n = 2, mean + SEM, at least 80 nuclei per condition and experiment).
  5. Western blot analysis of Flag‐histone H1 denaturing‐IP, showing ubiquitination pattern of histone H1 after 30 min of IR (10 Gy) treatment under indicated siRNA‐depleted conditions.
  6. Representative IF images showing the average intensity of endogenous BRCA1 at UV‐A micro‐laser‐induced DNA damage tracks in U2OS cells. Scale bar: 10 μm.
  7. Graph represents the quantification of (F) (ns P > 0.05, ***P < 0.001; unpaired t‐test, n = 2, mean + SEM, at least 100 nuclei per condition and experiment).

Source data are available online for this figure.