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A, B
BMDMos from cGAS−/− mice exhibit enhanced DNA repair efficiency than those from WT and Sting−/− mice. (A) Representative comet tails of WT, cGAS−/−, and Sting−/−BMDMos exposed to γ‐irradiation (IR: 10 Gy) on ice, then incubated at 37°C for indicated duration. (B) Corresponding quantification of the comet tail moments from 20 different fields with n > 200 comets of three independent experiments.
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C, D
cGAS promotes micronucleus generation in BMDMos independently of STING. (C) Confocal microscopic visualization of micronucleus (indicated by arrowhead) and cGAS staining in WT, cGAS−/−, and Sting−/− BMDMos exposed to γ‐irradiation (10 Gy). Scale bar: 10 μm. (D) Average MNs/cell in corresponding representative images. Bar graphs show mean values from eight different microscopic fields with over 200 cells.
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E, F
γ‐Irradiation‐induced cell death in WT and cGAS−/− BMDMos (E). γ‐Irradiation‐induced cell death in Sting−/− and cGAS−/− Sting−/− BMDMos (F).
Data information: Data are presented as mean ± SEM of
n = 3 independent experiments. Statistical significance was assessed using two‐way ANOVA in (B) and one‐way ANOVA in (D), (E), and (F) followed by Sidak's post‐test. *
P < 0.05, **
P < 0.01, ***
P < 0.001, and ****
P < 0.0001.
Source data are available online for this figure.
