Figure 5. DNA binding and subsequent oligomerization of cGAS are essential for inhibition of HR‐DNA repair.

• A, B
  Schematics of HR and NHEJ reporter assays.
• C, D
  Obtained results showing enhanced HR efficiency upon knockdown of endogenous cGAS in U2OS cells. Immunoblot inserts depict knockdown efficiency of cGAS and histone H1.2.
• E, F
  Results showing the effect of hcGAS, hcGAS▵cGAMP, hcGAS▵DNA, or hcGAS▵Oligo on HR (E) or NHEJ (F) in HEK293 cells. Corresponding immunoblot inserts depict cGAS expression.

Data information: Data are means with SEM, n = 3. Statistical significance was assessed using one‐way ANOVA followed by Sidak's post‐test. NS: P > 0.05, *P ≤ 0.05, ****P ≤ 0.0001.Source data are available online for this figure.