Figure EV5. cGAS inhibits RAD51‐mediated DNA strand exchange and D‐loop formation.

• A
  Coomassie Blue staining of purified hcGAS^cat, mcGAS^cat, Rad51, HOP2, and MND1.
• B
  Schematics of the D‐loop assay.
• C
  Pre‐incubation of template dsDNA with cGAS blocks subsequent D‐loop formation regardless of the presence of cGAMP precursors (ATP+GTP).
• D
  Schematics of the strand exchange reaction.
• E–H
  Pre‐incubation of dsDNA with cGAS protein inhibited the DNA strand exchange activity of human RAD51 (E, F) and yeast Rad51 (G, H) regardless of the presence of precursors (ATP+GTP) of cGAMP. The percentage of DNA strand exchange in each reaction was graphed as the average of triplicates ± SD.

Data information: Statistical significance was assessed using one‐way ANOVA followed by Sidak's post‐test. NS: P > 0.05, ****P ≤ 0.0001.Source data are available online for this figure.