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. 2019 Sep 23;38(21):e102718. doi: 10.15252/embj.2019102718

Figure 8. cGAS compacts dsDNA and inhibits D‐loop formation via oligomerization.

Figure 8

  1. Negative‐stain electron micrographs of cGAS‐dsDNA complexes following incubation of dsDNA with indicated cGAS variants. Scale bar: 100 nm.
  2. Effect of indicated hcGAS variants on D‐loop formation when pre‐incubated with dsDNA.
  3. Percentage of D‐loop formed in each reaction (left) graphed as the average of triplicates ± SD.
  4. Overview of a single 1:1 hcGAS‐DNA complex depicting the location of the Y215 within the cGAS‐dsDNA interface.
  5. DR‐GFP assay showing that hcGASΔDNA‐Y215E is impaired in HR inhibition.
  6. hcGASΔDNA‐Y215E but not hcGASΔcGAMP has a decreased affinity to dsDNA24.
  7. hcGASΔDNA‐Y215E and hcGASΔcGAMP are defective in synthase activity.
  8. Negative‐stain electron micrographs showing that hcGAScat‐ΔDNA‐Y215E is defective in inducing cGAS‐dsDNA complexes. Scale bar: 100 nm.
  9. Effect of indicated hcGAS variants on D‐loop formation.
Data information: Data are means ± SD, n = 3. Unpaired Student's t‐test was used for statistical analyses: NS P > 0.05, **P ≤ 0.01, ***P ≤ 0.001, and ****P ≤ 0.0001.Source data are available online for this figure.