Figure 1. Monoclonal and polyclonal anti‐AdV antibodies trigger inflammasome activation in human macrophages.

• A
  THP‐1 cells were stimulated with (50,000 particles/cell) AdV‐GFP and infection after 24 h measured by flow cytometry.
• B,C
  Cells were stimulated as in (A) with AdV‐GFP or AdV‐GFP complexed with 20 μg/ml h9C12 or 20 mg/ml human IVIg. Cell supernatants were harvested at indicated time‐points, and cytokines measured by ELISA (B) or pro‐ and cleaved IL‐1β were measured in whole cell lysates or in supernatants by Western blot (C). Data are representative of two independent experiments. (D) HMDM were primed with 10 μg/ml pI:C for 2 h and then stimulated for 16 h with virus +/− antibody. Cytokines in the supernatants were measured by ELISA (n = 7, mean ± s.e.m. *P ≤ 0.05, **P ≤ 0.005, paired, two‐tailed t‐test).

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