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. Author manuscript; available in PMC: 2019 Nov 4.
Published in final edited form as: Genesis. 2019 Mar 28;57(6):e23294. doi: 10.1002/dvg.23294

FIGURE 3.

FIGURE 3

Characterization of Nppa-tagBFP knock-in reporter expression during heart development using immunostaining. Frozen sections of the whole embryos and/or hearts at E9.5, E12.5, and E15.5 were processed. The frozen sections were immunostained for a cardiomyocyte marker, cardiac troponin-T (cTnT), and tagBFP. Immunostained frozen sections of whole embryo at E9.5 (a) showed tagBFP expression in the ventricle. Magnified view of heart area at E9.5 demonstrated ventricular specific tagBFP expression (b). Embryonic heart sections at E12.5 (e) and E15.5 (f) showed tagBFP expression in all four chambers. The ventricular walls indicated by the white inlets at E9.5, E12.5, and E15.5 were magnified (g–i). A: atrium; V: ventricle; IFT: inflow tract; OFT: out flow tract; AVC: atrioventricular canal; LV: left ventricle; RV: right ventricle; LA: left atrium: RA; right atrium