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. 2019 Oct 15;8(10):bio047126. doi: 10.1242/bio.047126

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — HS in progenitors restricts ISC proliferation. (A) esg⁺ cells (green) in control flies at 29°C for 10 days (white arrowheads). (B,C) The number of esg⁺ cells (green) is dramatically increased in esg^ts>sfl^RNAi flies at 29°C for 10 days (white arrowheads). (D) The number of esg⁺ cells (green) is dramatically increased in esg^ts>sgl^RNAi flies at 29°C for 10 days (white arrowheads). (E) The number of esg⁺ cells (green) is dramatically increased in esg^ts>ttv^RNAi flies at 29°C for 10 days (white arrowheads). (F) The number of esg⁺ cells (green) is dramatically increased in esg^ts>sotv^RNAi flies at 29°C for 10 days (white arrowheads). (G,H) The number of esg⁺ cells (green) is dramatically increased in esg^ts>botv^RNAi flies at 29°C for 10 days (white arrowheads). (I) Quantification of the relative number of esg⁺ cells in the different genotypes indicated. mean±s.d. is shown. n=10–15 intestines. **P<0.01. (J) Quantification of the number of pH3 per gut in the different genotypes indicated. mean±s.d. is shown. n=10–15 intestines. **P<0.01. In all panels except graphs, GFP is in green and blue indicates DAPI staining for DNA. Scale bars: 20 μm.