Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 20;8(10):bio045724. doi: 10.1242/bio.045724

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 3. — Cell dynamics during the epithelial repair process upon luminal cell ablation. (A) Timeline of experiments of luminal cell-specific ablation. (B) IF and TUNEL staining 1 day after tamoxifen induction. Scale bar: 20 μm. (C) H&E images showing 1 day and 14 days prostate epithelium. Scale bar: 50 μm. (D) IF images showing proliferating cells in basal and luminal cells at different time points of tissue repair. Scale bar: 20 μm. (E) Quantitation of Ki67 index in the basal and luminal cell populations at different time points of tissue repair. (F) IF image showing basal cell over-proliferation and CK5⁺CK18⁺ intermediate cells (arrow) at 1 day. Scale bar: 20 μm. (G) Comparison of intermediate cell percentage between 1 day and wild-type control. (H) Quantitation of basal and luminal cell proliferation by BrdU incorporation assay during tissue repair. Error bars in E,G,H correspond to one s.d. N=3 animals per time point. **P<0.001; *P<0.01 by two-tailed Student's t-test.