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. 2019 Oct 1;42(6):2355–2362. doi: 10.3892/or.2019.7350

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Copyright: © Xu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Verapamil increases TMZ-induced tumor sphere formation and apoptosis in TMZ-resistant rat glioma (C6-PL3) and parental rat glioma (C6) cells. (A) Tumor sphere formation in C6-PL3 and C6 cells was observed using phase-contrast microscopy after TMZ and verapamil treatment. (B and C) After treatment, apoptosis was detected using flow cytometry. The average apoptosis rate of TMZ treatment was 4.79%, while after adding the verapamil, the average apoptosis rate was 81.61%. ***P<0.001. TMZ, temozolomide.