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. 2019 Oct 1;42(6):2486–2498. doi: 10.3892/or.2019.7348

Figure 2.

Figure 2.

Ku80, RET/TC and NF-κB p65 expression in NTT, TC, HT and paracancerous tissues. (A) IHC staining for Ku80 assessment in TC and adjacent tissues (magnification, ×100). Ku80 was expressed in TC tissues (strong positive staining of nuclei) but not in adjacent tissues (no positive staining of nuclei or cytoplasm). (B) IHC detection of RET/TC protein expression in the diseased thyroid tissue (magnification, ×40). HT exhibited weak positive staining of the cytoplasm; TC exhibited strong positive staining of the cytoplasm; TC with paraneoplastic (slightly weakly positive staining of cytoplasm; NTT exhibited negative staining of the cytoplasm and nuclei. (C) Assessment of NF-κB p65 protein expression in the diseased thyroid tissue by IHC (magnification, ×40). HT exhibited weak positive cytoplasmic and nuclear staining; TC exhibited strong positive cytoplasmic and nuclear staining; NTT exhibited almost negative staining of the cytoplasm or nuclei. (D) Immunofluorescence double-labeling and laser confocal microscopy for RET/TC antigen and NF-κB detection (magnification, ×600). NF-κB expression is shown as red fluorescence, RET/TC antigen expression is shown as green fluorescence. Laser confocal microscopy results show NF-κB and RET/TC were co-expressed in the cytoplasm and/or nuclei of thyroid follicular epithelial cells. TC, thyroid carcinoma; HT, Hashimoto's thyroiditis; NTT, normal thyroid tissue; IHC, immunohistochemistry; NF-κB, nuclear factor-κB.