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Schematic diagram of CRISPR/Cas9. The CRISPR/Cas9 dual-vector lentivirus knocked out the target sequence of the Ku80 gene. CRISPR, clustered regularly interspaced short palindromic repeats; Cas9, CRISPR-associated protein 9; tracrRNA, trans-activating RNA; crRNA, CRISPR-derived RNA; PAM, proto-spacer adjacent motifs.
