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. 2019 Oct 10;42(6):2537–2549. doi: 10.3892/or.2019.7365

Figure 4.

Figure 4.

Impaired lipid metabolism function in cocultured adipocytes is accompanied by insulin resistance. Immunoblots and quantification of key proteins associated with lipid metabolism in adipocytes cultured alone, and cocultured with (A) Panc-1 or with (B) Mia PaCa2. (C) The mature adipocytes were cultivated with/without Panc-1 for 5 days before incubation with 100 nM insulin for 30 min. The expression of total and p-Akt was analyzed via western blotting. (D) Reverse transcription-quantitative PCR analysis of Glut4 and IRS-1 mRNA expression in mature adipocytes grown with/without Panc-1 for 5 days. Data are presented as the mean ± standard deviation. *P<0.05; **P<0.01. p-Akt, phosphorylated Akt.