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. 2019 Oct 14;10(10):805. doi: 10.3390/genes10100805

Figure 2.

Figure 2

The overexpression of miR-106a promotes porcine preadipocyte proliferation. The miR-106a agomir or negative control (NC) were transfected into cells, and cells were harvested 48 h after transfection. (A,B) 5-Ethynyl-20-deoxyuridine (EdU) staining assayed the number of proliferous cells; positive cells were stained by EdU (red) and total cell nucleus was stained with Hoechst (blue); (C) the cell count was measured by cell count kit-8 (CCK-8), and the results illustrated the absorbance value at 450 nm, after incubation with 10% CCK-8 solution for 4 hours; (D) the overexpression efficiency of miR-106a after transfection with the miR-106a agomir compared with NC; (E) western blot analysis of cell cycle genes; (F) quantification of the western blot analysis of cyclin B, cyclin D, cyclin E and proliferating cell nuclear antigen (PCNA); (G,H) cell cycle analysis was performed by a flow cytometer; (I) the statistical results of cell cycle analysis. Data are representative of the mean ± SEM of three independent experiments; scale bar: 100 μm; * p < 0.05, ** p < 0.01.