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. 2019 Oct 14;10(10):805. doi: 10.3390/genes10100805

Figure 4.

Figure 4

The overexpression of miR-106a accelerates porcine preadipocyte differentiation. The miR-106a agomir or NC were transfected into cells at 80% density at 50 nmol/L; (A) boron dipyrromethene (BODIPY) staining was performed in cells on the 8th day of differentiation, the lipid droplets were stained with BODIPY (green) and the cell nucleus was stained with DAPI (blue). Oil Red O staining was performed at day 6 after adipogenic differentiation; (B) triglycerides content was measured by spectrophotometric analysis at 490 nm; (C) the overexpression efficiency of miR-106a after transfection with the miR-106a agomir; (D–F) real-time qPCR (RT-Qpcr) was used to detect adipogenesis genes, CCAAT/enhancer-binding protein β (C/EBPβ), peroxisome proliferator- activated receptor γ (PPARγ), and fatty acid binding proteins (aP2) on the 2nd day, 6th day, and 8th day after adipogenic induction; (G) western blot analysis of the adipogenic genes’ protein expression on the 2nd day and 8th day after adipogenic induction; (H) the quantification of adipogenic genes protein expression levels. Data are representative of the mean ± SEM of three independent experiments. Scale bar: 100 μm; * p < 0.05, ** p < 0.01.