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. 2019 Oct 22;11(10):1613. doi: 10.3390/cancers11101613

Figure 4.

Figure 4

Figure 4

MIR452 regulated VEGFA-mediated VEGFR2–SRC signaling in CRC cells. (A) Western blot analysis of VEGFR2-regulated proteins, SRC and protein tyrosine kinase 2 (PTK2), in Caco2 cells. (B) Western blot analysis of SRC and PTK2 in SW48 cells. Three independent experiments were performed in duplicate, and the p-values were calculated using Student’s t-test (** p < 0.01). (C) The scratch wound assay was conducted using HT29 cells transfected with the MIR452 mimic, siVEGFA, or mock controls. The migration distance was measured 0, 48, and 96 h after the cells were scratched. Three independent experiments were performed in duplicate, and the p-values were calculated using Student’s t-test (** p < 0.01). (D) For the migration assay, we used 24-well Transwell chambers, which separated the upper and lower compartments by polycarbonate membranes that consisted of 8 μm pores. The cells that retained the dye were quantified by measuring absorbance at 560 nm (A560). Compared with the mock control, the MIR452 mimic reduced migration of both Caco2 and HT29 cells. Three independent experiments were performed with duplicates, and the p-values were calculated using Student’s t-test (* p < 0.05).