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. 2019 Oct 22;11(10):1613. doi: 10.3390/cancers11101613

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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MIR452 regulated angiogenesis by extracellular VEGFA secretion. (A) The extracellular VEGFA levels in Caco2 and SW48 cells transfected with the MIR452 mimic, siVEGFA, or mock controls. Three independent experiments were performed in duplicate, and the p-values were calculated using Student’s t-test (** p < 0.01). (B) Rat aortic ring angiogenesis assay. The aortic rings from four-week-old Sprague–Dawley rats were randomly seeded onto Matrigel-coated wells and sealed with an overlay of Matrigel. They were co-cultured with Caco2 or SW48 cells transfected with the MIR452 mimic (upper panel), siVEGFA (bottom panel), or mock controls. After 7 days, microvessel sprouting was photographed using an inverted microscope (Olympus; 2.5× magnification). Four independent experiments were performed in duplicate.