Figure 5.

Sequence divergence among the 97 L. major assembled minicircles (circular) inferred by evolutionary analysis. The analysis was carried using the maximum likelihood method and Tamura-Nei model [39]. The tree with the highest log likelihood (–67786.96) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches (bootstrap values were obtained from 500 replicates). Initial tree(s) for the heuristic search were obtained automatically by applying the neighbor-join (NJ) and BioNJ algorithms to a matrix of pairwise distances estimated using the maximum composite likelihood (MCL) approach, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. There were 936 positions in the final dataset.