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Differentiation of chicken preadipocytes. (A) Differentiated preadipocytes before oil red o staining. (B) Differentiated preadipocytes stained using Oil red O. (C) The relative expression level of adipocyte marker genes before and after differentiation. The relative expression level of FABP4 and PPARG genes significantly increased post-differentiation, indicating that the chicken preadipocytes were successfully induced. *denotes p < 0.05, **denotes p < 0.01.
