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. 2019 Oct 17;11(10):1583. doi: 10.3390/cancers11101583

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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CD200 overexpression enabled MEER tumors to metastasize to DLNs. (A) One million MEER cells were subcutaneously implanted into C57BL6 mice. When the tumors were approximately of 200–300 mm³, the tumors, DLNs, and major organs were harvested. CD200 expression was analyzed to confirm expression differences between MEER/CD200^High and MEER/CD200^High tumors. The DLNs were sectioned to detect metastasis using an anti-CK18 antibody, and the numbers of metastases were histologically evaluated. (B) The frequency of metastasis in mouse DLNs was 100% (8 mice per group). DLNs were sectioned to count metastatic cells and were immunostained for CK18+. The metastatic cells from MEER/CD200^High tumors were histologically scored compared to metastatic CK18+ cells of MEER/CD200^Low tumors, which were assigned a metastatic score of 1. The bar in macroscopic image indicates 100 μm.