Figure 2.

Expression pattern of porcine Prox1 at different developmental stages. Longissimus dorsi muscles were collected from three fetuses of Landrace pregnant females at 70 days (P70) and 110 days (P110) post conception (dpc), and from three pigs on day 1 (D1) and at 70 days (D70) after birth. Real-time PCR was performed to determine Prox1 expression levels. All PCR reactions were performed in triplicate for each sample. The relative expression levels were calculated using the 2^−△△ct value method and normalized with porcine housekeeping gene HPRT. Statistical analysis was performed using one-way ANOVA with Duncan’s multiple range tests. Data are presented as the mean ± SEM (n = 3). Different letters above the bars indicate significant differences. Note: p < 0.05.