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. 2019 Oct 12;11(10):1541. doi: 10.3390/cancers11101541

Figure 6.

Figure 6

A five-day running schedule fully protects gastrocnemius from wasting in C26-bearing mice, reversing musclin reduction in muscle and plasma. C26 tumor size is not affected by five-day running in mice (a), n = 10. The gastrocnemius weight is fully preserved in C26-bearing mice in the running schedule (b). One-way ANOVA followed by Tukey’s test, **** p ≤ 0.0001, n = 4–9. C26-induced atrogin-1 expression in gastrocnemius is restrained in the running-trained mice (c). While running has no effect on the expression of FGF21 (d) or FNDC5 (e), the expression of musclin in gastrocnemius (f) and the plasma concentration of musclin (g) are respectively partially and fully recovered in five day-trained mice. IPO8 was used as housekeeping gene. One-way ANOVA followed by Tukey’s test, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001 n = 8–10. In WB, the protein content of PGC1α, musclin and its receptor Npr3 in gastrocnemius of C26-bearing mice is restored to levels comparable to tumor-free mice after five-day running (h). Densitometric analysis is shown for PGC1α (i), musclin (l) and Npr3 (m). Vinculin served as loading control. One-way ANOVA followed by Tukey’s test, * p ≤ 0.05, ** p ≤ 0.01, n = 3.