Table 5.
Items3 | Met Source | Met Dose | CAT (U/mg-prot) | T-SOD (U/mg-prot) | GSH-Px (U/mg-prot) | T-AOC (U/mg-prot) | MDA (nmol/mg-prot) |
---|---|---|---|---|---|---|---|
1 | BD | 0 | 0.70 | 20.54b | 31.84b | 0.40 | 0.84a |
2 | dl-Met | 0.15% | 0.79 | 24.36ab | 37.81ab | 0.44 | 0.78ab |
3 | dl-Met | 0.30% | 0.79 | 27.28a | 39.99a | 0.44 | 0.69bc |
4 | dl-Met | 0.45% | 0.78 | 26.98a | 39.25a | 0.43 | 0.70bc |
5 | dl-Met | 0.60% | 0.81 | 28.29a | 38.96a | 0.46 | 0.66c |
6 | dl-Met-Met | 0.15% | 0.77 | 26.20a | 39.01a | 0.45 | 0.72bc |
7 | dl-Met-Met | 0.30% | 0.80 | 28.66a | 43.11a | 0.44 | 0.70bc |
8 | dl-Met-Met | 0.45% | 0.79 | 28.38a | 41.17a | 0.45 | 0.70bc |
9 | dl-Met-Met | 0.60% | 0.84 | 28.36a | 42.43a | 0.44 | 0.66c |
SEM | 0.010 | 0.643 | 0.808 | 0.008 | 0.013 | ||
P-values | |||||||
treatment effects | 0.104 | 0.034 | 0.032 | 0.926 | 0.019 | ||
Met source | 0.707 | 0.324 | 0.147 | 0.813 | 0.689 | ||
main effect of the Met source | |||||||
dl-Met | 0.79 | 26.73 | 39.25 | 0.44 | 0.71 | ||
dl-Met-Met | 0.80 | 27.90 | 41.43 | 0.45 | 0.70 | ||
linear effect4 | |||||||
dl-Met | 0.025 | 0.004 | 0.048 | 0.296 | 0.005 | ||
dl-Met-Met | 0.015 | 0.003 | <0.001 | 0.305 | 0.005 | ||
quadratic effect4 | |||||||
dl-Met | 0.163 | 0.216 | 0.061 | 0.668 | 0.202 | ||
dl-Met-Met | 0.445 | 0.029 | 0.012 | 0.277 | 0.176 |
1 Values without the same small letters in the column indicate a significant difference (p < 0.05, n = 8). The data are presented as the mean ± standard error; 2 CAT: catalase; T-SOD: total superoxide dismutase; GSH-Px: glutathione peroxidase; T-AOC: total antioxidant capacity; MDA: malonaldehyde; 3 1: BD; 2: 0.15% dl-Met; 3: 0.30% dl-Met; 4: 0.45% dl-Met; 5: 0.60% dl-Met; 6: 0.15% dl-Met-Met; 7: 0.30% dl-Met-Met; 8: 0.45% dl-Met-Met; 9: 0.60% dl-Met-Met. (BD: basal diet; dl-Met: dl-methionine; dl-Met-Met: dl-methionyl- dl-methionine); 4 Trend analysis for BD and BD with 0.15%, 0.30%, 0.45% and 0.60% dl-Met or dl-Met-Met.