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. 2019 Sep 28;11(10):1457. doi: 10.3390/cancers11101457

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Overexpression of miR-34a suppresses tumorigenicity and drug resistance in UMUC3 UC cells. (A) Cell proliferation of UMUC3 cells stably expressing miR-34a or an empty vector was measured using cell counting kits (CCKs), on the designated days. Anchorage-independent growth in UMUC3 cells overexpressing miR-34a was determined using soft-agar (B) and cell invasion (magnification 100×) (C) assays. Quantitative analysis of the number of colonies in the soft agar and invasion assays indicated below. (D) Chemosensitivity to cisplatin in UMUC3 cells overexpressing miR-34a, as determined using a CCK. (E) Nude mouse xenograft model showing tumor sizes of UMUC3 cells overexpressing miR-34a (red line) or a control plasmid (blue line). Representative examples of nude mice bearing tumors are also presented (left: miR-34a, red arrow head; right: control, blue arrow head). * p < 0.05; ** p < 0.01, *** p < 0.001.

Overexpression of miR-34a suppresses tumorigenicity and drug resistance in UMUC3 UC cells. (A) Cell proliferation of UMUC3 cells stably expressing miR-34a or an empty vector was measured using cell counting kits (CCKs), on the designated days. Anchorage-independent growth in UMUC3 cells overexpressing miR-34a was determined using soft-agar (B) and cell invasion (magnification 100×) (C) assays. Quantitative analysis of the number of colonies in the soft agar and invasion assays indicated below. (D) Chemosensitivity to cisplatin in UMUC3 cells overexpressing miR-34a, as determined using a CCK. (E) Nude mouse xenograft model showing tumor sizes of UMUC3 cells overexpressing miR-34a (red line) or a control plasmid (blue line). Representative examples of nude mice bearing tumors are also presented (left: miR-34a, red arrow head; right: control, blue arrow head). * p < 0.05; ** p < 0.01, *** p < 0.001.