Table 2.
Summary of NGS platforms and gene panels used in the reviewed studies.
| Ref. | Company | Platform | Gene Panel | Number of Tested Genes | Library Preparation Protocols |
|---|---|---|---|---|---|
| [60] | Life Technologies | Ion PGM | Custom panel | 12 | NR |
| [54] | 21 | Ion AmpliSeq Library Kit 2.0 | |||
| [56] | SV-CA50-ctDNA panel | 50 | Ion AmpliSeq Library Kit 2.0 | ||
| [59] | Ion AmpliSeq Cancer Hotspot panel v2 | 50 | Ion AmpliSeq Kit | ||
| [51] | Ion AmpliSeq Cancer Hotspot panel v2 | 50 | Ion AmpliSeq Library Kit Plus | ||
| [50] | Not reported | 22 | Oncomine Solid Tumor DNA Kit | ||
| [61] | Ion AmpliSeq Colon & Lung v2 | 22 (KRAS) * | Oncomine Solid Tumor DNA Kit | ||
| [55] | Ion Proton | 22 | NR | ||
| [62] | Ion S5 | Custom panel | 14 | Oncomine Colon cfDNA | |
| [45] | Illumina | MiSeq | Custom panel | 15 (single mutation) * | NR |
| [58] | SOMATIC 1 MASTRTM v2 | BRAF, KRAS, NRAS | NR | ||
| [66] | MiSeq | Accu-Act Panel | 61 | CRC01 | |
| [52] | HiSeq 2500 | NCC Oncopanel | 90 | KAPA Hyper-prep | |
| [57] | Custom assay | 40 (KRAS, NRAS, HRAS, BRAF) * | SureSelect QTX | ||
| [44] | NextSeq 500 | AVENIO ctDNA Expanded Kit | 77 | Custom | |
| [53] | Custom assay | 18 | NR | ||
| [48] | NR | PlasmaSelect-R | 63 | NR | |
| [49] | Guardant Health | Guardant360 | 54 | Hybrid capture | |
| [64] | 54, 68, or 70 | ||||
| [65] | 54–73 | ||||
| [63] | 73 | ||||
| [67] | Guardant Health | Guardant360 | Ion AmpliSeq 2.0 | 68 | |
| Thermo Fisher Scientific | Ion Torrent | Oncomine Comprehensive Cancer Panel | 143 | ||
* In parentheses, the genes the status of which was of interest to the study. Ref, reference. NR, not reported.