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. 2019 Oct 19;9(10):284. doi: 10.3390/brainsci9100284

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of macrophage migration inhibitory factor (MIF) and D-dopachrome tautomerase (DDT) inhibition on vincristine resistance. The human neuroblastoma cell line UKF-NB-3 adapted to 10 ng/mL vincristine (VCR10) was transfected with double-stranded siRNAs (dsRNA) targeting the MIF gene (NM_002415) (A) or the DDT gene (NM_001084392) (B), and corresponding scrambled siRNAs, and exposed to 5 scalar ten-fold concentrations of vincristine (range: 0.01–100 ng/mL). Cell viability was tested by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) dye reduction assay after 48 h of incubation. The parental UFK-NB-3 cell line was used as control. All experiments were performed in triplicate.