Figure 4.

Quality report by 1% agarose gel electrophoresis. The marker is Trans 2K Plus. The figure corresponds to the following sequences: sequences 1, 2, 7, and 11 were diluted 20-fold; sequences 4, 8–10, 12–14, and 16 were diluted 10-fold; and the samples were 1 μL. Sequence 5 was diluted sevenfold, and the sample was 1 μL. Test parameters: gel concentration, 1%; voltage, 180 v; and electrophoretic time, 16 min. Agarose imaging was no less than three bands: 28 S, 18 S, and 5 S; the 28 S and 18 S bands were clear, and the brightness of 28 S was more than twice that of 18 S. The integrity of total RNA was better.