Table 2.
Diagnostic performance of individual protein biomarkers for detecting CRC.
| Protein Biomarkers | Discovery Set (LC/MRM-MS Measurements) | Discovery Set (PEA Measurements) | Validation Set (PEA Measurements) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| p-val | p-valadj | AUC (95% CI) |
AUCBS (95% CI) |
Se% at 90% Sp | p-val | p-valadj | AUC (95% CI) |
AUCBS (95% CI) |
Se% at 90% Sp | p-val | p-valadj | AUC (95% CI) |
AUCBS (95% CI) |
Se% at 90% Sp | |
| CDH5 | 0.35 | 0.38 | 0.54 (0.46–0.62) |
0.48 (0.36–0.61) |
8 | 0.20 | 0.25 | 0.55 (0.47–0.64) |
0.50 (0.36–0.64) |
10 | <0.05 | <0.05 | 0.62 (0.54–0.71) |
0.59 (0.50–0.73) |
12 |
| Gal 3 | 0.51 | 0.51 | 0.53 (0.45–0.61) |
0.47 (0.36–0.59) |
7 | 0.84 | 0.84 | 0.51 (0.43–0.59) |
0.46 (0.37–0.55) |
7 | 0.13 | 0.16 | 0.57 (0.48–0.67) |
0.51 (0.33–0.67) |
11 |
| IGFBP2 | <0.05 | <0.05 | 0.61 (0.53–0.69) |
0.58 (0.50–0.71) |
24 | <0.05 | <0.05 | 0.61 (0.53–0.69) |
0.58 (0.50–0.72) |
21 | 0.43 | 0.46 | 0.54 (0.44–0.63) |
0.48 (0.34–0.62) |
10 |
| MASP1 | <0.001 | <0.001 | 0.68 (0.61–0.76) |
0.67 (0.58–0.78) |
27 | <0.001 | <0.001 | 0.65 (0.57–0.72) |
0.63 (0.53–0.75) |
20 | 0.08 | 0.11 | 0.58 (0.49–0.68) |
0.53 (0.37–0.68) |
13 |
| MMP9 | 0.24 | 0.33 | 0.55 (0.47–0.63) |
0.50 (0.37–0.62) |
10 | 0.32 | 0.36 | 0.55 (0.38–0.54) |
0.50 (0.38–0.62) |
10 | 0.05 | 0.08 | 0.59 (0.50–0.69) |
0.55 (0.46–0.71) |
16 |
| MPO | 0.06 | 0.11 | 0.58 (0.50–0.66) |
0.47 (0.36–0.59) |
8 | <0.005 | <0.005 | 0.63 (0.55–0.71) |
0.60 (0.52–0.73) |
17 | 0.53 | 0.53 | 0.53 (0.44–0.62) |
0.46 (0.35–0.57) |
6 |
| OPN | <0.001 | <0.001 | 0.64 (0.57–0.72) |
0.62 (0.54–0.75) |
26 | <0.001 | <0.001 | 0.75 (0.68–0.82) |
0.73 (0.65–0.84) |
35 | <0.05 | <0.05 | 0.62 (0.53–0.71) |
0.59 (0.49–0.73) |
18 |
| PON3 | <0.001 | <0.001 | 0.73 (0.66–0.80) |
0.72 (0.63–0.82) |
32 | <0.001 | <0.001 | 0.75 (0.68–0.82) |
0.74 (0.66–0.84) |
43 | 0.05 | 0.08 | 0.59 (0.51–0.68) |
0.54 (0.37–0.69) |
11 |
| PRTN3 | 0.13 | 0.21 | 0.56 (0.48–0.64) |
0.51 (0.36–0.65) |
10 | <0.001 | <0.001 | 0.64 (0.56–0.72) |
0.61 (0.54–0.74) |
18 | 0.06 | 0.09 | 0.59 (0.50–0.68) |
0.49 (0.31–0.65) |
5 |
| SPARC | 0.30 | 0.37 | 0.54 (0.46–0.63) |
0.49 (0.37–0.61) |
10 | 0.33 | 0.36 | 0.54 (0.46–0.62) |
0.49 (0.36–0.62) |
9 | 0.41 | 0.46 | 0.54 (0.45–0.63) |
0.48 (0.36–0.61) |
5 |
| TR | <0.001 | <0.001 | 0.67 (0.60–0.75) |
0.66 (0.57–0.77) |
35 | <0.001 | <0.001 | 0.70 (0.63–0.77) |
0.69 (0.60–0.79) |
36 | <0.001 | <0.001 | 0.74 (0.66–0.82) |
0.72 (0.64–0.85) |
33 |
| AREG | - | - | - | - | - | <0.001 | <0.001 | 0.79 (0.73–0.86) |
0.78 (0.70–0.87) |
54 | <0.001 | <0.001 | 0.72 (0.64–0.80) |
0.70 (0.61–0.83) |
35 |
Abbreviations: AUC—area under the receiver operating curve; AUCBS—.632+ bootstrap estimates of AUC; CRC—colorectal cancer; 95% CI—95% confidence interval; LC/MRM-MS—liquid chromatography/multiple reaction monitoring-mass spectrometry; PEA—proximity extension assay; Se—sensitivity; Sp—specificity. All proteins abbreviations: AREG—amphiregulin; CDH5—cadherin 5; Gal 3—galectin 3; IGFBP2—insulin like growth factor binding protein 2; MASP1—mannan binding lectin serine protease 1; MMP9—matrix metalloproteinase 9; MPO—myeloperoxidase; OPN—osteopontin; PON3—serum paraoxonase lactonase 3; PRTN3—myeloblastin; SPARC—SPARC protein; TR—transferrin receptor protein 1.