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. 2019 Oct 19;11(10):1597. doi: 10.3390/cancers11101597

Figure 4.

Figure 4

Maturation and viability of DCs after coculture with pPBS-treated PSCs and PCCs. (a) Box plot from minimum to maximum value of ΔMFI of the maturation marker CD86. CD86 expression is examined on immature DCs after 48 h of coculture of pPBS-treated PCCs and PSCs (effector/target (E/T) ratio, 1:1), and after pPBS treatment on immature DCs without coculture using flow cytometry. ΔMFI represents [(MFI staining treated–MFI isotype treated)–(MFI staining untreated–MFI isotype untreated)]. Treatment of 50% pPBS is used for MIA-Paca-2 and Capan-2, while treatment of 100% pPBS is used for PANC-1, BxPC3, hPSC128, hPSC21, and RLT-PSC. Every dot represents a different healthy donor and ≥3 donors were used per cell line. * p < 0.05 significant differences compared with untreated control. (b) Percentage of viability of DCs after 48 h coculture with pPBS-treated PCC lines (MIA-Paca-2, PANC-1, BxPC3, Capan-2) and PSC lines (hPSC128, hPSC21, RLT-PSC) or pPBS treatment alone. Graph represent mean of ± SEM of ≥3 independent experiments with different donors. * p < 0.05 significant differences compared with untreated control.