Skip to main content
. 2019 Oct 2;11(10):1488. doi: 10.3390/cancers11101488

Figure 5.

Figure 5

Nrf2 promotes an epithelial phenotype and antagonizes the mesenchymal state. (A) Nrf2 knockdown in A549 and HCC4006 leads to an increase in mesenchymal marker expression and a decrease in epithelial marker expression, as well as a decrease in FASN and increase in pERK by immunoblot. (B) Nrf2 knockdown in A549 and HCC4006 decreases cell proliferation over 72 h (p < 0.05 *; p < 0.01 **; p < 0.001 ***). (C) Nrf2 Knockdown in A549 and HCC4006 causes loss of E-cadherin (red) from the cell membrane by IF, where nuclei are stained with DAPI (blue). (D) When Nrf2 is overexpressed in HCC4006, mesenchymal marker expression decreases (N-cad) while the epithelial marker (E-cad) increases by immunoblot. (E) In HCC4006, treatment with 1, 3, 5 mM NAC over the course of 72 h, a loss of M markers and gain of E markers is seen in the M state.