. 2019 Oct 21;9(10):847. doi: 10.3390/ani9100847

Table 2.

The primer sequences for qRT-PCR of antioxidant relative mRNA expression of jejunum and ileum mucosa and liver in weaned pigs.

Genes	Orientation	Primer Sequence 5′–3′	Size (bp)	Tm (°C)
GAPDH	Forward	CTGCCGCCTGGAGAAACCT	226	60
GAPDH	Reverse	GCTGTAGCCAAATTCATTGTCG	226	60
SOD1	Forward	GAGACCTGGGCAATGTGACTG	190	60
SOD1	Reverse	GCCAAACGACTTCCAGCAT	190	60
GPX1	Forward	CAGGCGGCGGGTTCG	129	60
GPX1	Reverse	TGAGGGCAGTGGCATCGT	129	60
GPX4	Forward	GAAGGACCTGCCGTGCTACC	159	60
GPX4	Reverse	CCTTCCTCTGGCGGGGTT	159	60
CAT	Forward	CCCGCATTCAGGCTCTTC	116	60
CAT	Reverse	CCCTCACAGGTTAGCTTTCTCC	116	60

Tm = annealing temperature.