Figure 6.

Deuterium incorporation at the bis-allylic positions of the lead molecule IP-DHA do not impede cytoprotective effect against toxic all-trans retinal (25 µM) in ARPE-19 cells. ARPE-19 cells were incubated with IP-DHAs at increasing concentrations (0–80 µM) in serum free medium for 1 h, followed by the incubation of all-trans retinal at 25 µM during 4 h. After 20 h cell survival was determined using MTT assay. All data are presented as means ± SD (n = 3 independent experiments, each experiment in sextuplicate). The data are expressed as the percentage of untreated cells (CTL). Statistical analysis was performed using a Mann-Whitney test; * p < 0.05, ** p < 0.01, *** p < 0.001, versus all-trans-retinal treated cells (black rectangle).