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Dox uptake mechanism and efficacy test using the reactive oxygen species (ROS) assay kit. H2O2 was used as a positive control. (A) ROS assay fluorescence for MDA-MB-231 cells. (B) ROS assay fluorescence for HeLa cells. * indicates p-value < 0.05, ** indicates p-value < 0.01, and *** indicates p-value < 0.001. ns: no significant difference. Statistical analysis was done by using one-way ANOVA followed by post hoc analysis with Tukey’s multiple comparisons test.
