Figure 5.

PAK1 physically interacts with Stat3, PAK1 and Stat3 colocalize in the nucleus and PAK1 inactivation induces Stat3 dephosphorylation. (A) Total protein from breast cancer cells was immunoprecipitated with anti-PAK1 or anti-Stat3 antibodies, followed by immunoblotting using anti-Stat3 or anti-PAK1 antibodies. (B) Immunofluorescence analysis of PAK1 (green) and Stat3 (red) expression in breast cancer cells using the indicated antibodies. Images were captured by microscopy at 10× magnification. (C) Effect of ivermectin on the pStat3 level. (D) Immunofluorescence analysis of PAK1 (green) and Stat3 (red) expression levels in breast cancer cells after ivermectin treatment. Images were captured by microscopy at 10× magnification (scale bar = 100 μm).