Figure 3. A, Relative miR-129-5p expression was detected by qRT-PCR analysis in MCF-7 cells transfected with miR-129-5p mimics or miR-NC. MiR-NC acted as a negative control. B and C, Cells were transfected with miR-NC or miR-129-5p mimics and then treated with 31.2 nM Taxol for 24 h. B, LC3B-I, LC3B-II, and p62 expression in MCF-7 cells were determined by western blot. C, Cellular apoptosis was determined by flow cytometry. D, Cell proliferation was determined by the CCK-8 assay after transfection with miR-NC or miR-129-5p mimics and treatment with different concentrations of Taxol for 24 h. Data are reported as the means±SD of three independent experiments. *P<0.05, **P<0.01, vs miR-NC group; #P<0.05, ##P<0.01, vs miR-NC+Taxol group (ANOVA).
