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. 2019 Oct 2;11(10):1486. doi: 10.3390/cancers11101486

Figure 6.

Figure 6

Modulation between migration and invasion enhancer 1 (MIEN1) and interleukin-6 (IL-6) in prostate carcinoma PC-3 cells. Relative expression of MIEN1 and IL-6 after ectopic MIEN1 overexpression (A) or MIEN1 knockdown (B) in PC3 cells as determined by RT-qPCR assays. Data are presented as the mean fold induction of the mRNA levels (± standard error (SE), n = 3) relative to the mock-transfected groups PC-DNA and PC_shCOL, respectively. Secretion of IL-6 in MIEN1-overexpressing (C) or MIEN1-knockdown (D) PC3 cells. (E) Relative luciferase activity of IL-6 reporter vector after cotransfection with various dosages of MIEN1 expression vector (± SE, n = 6). (F) Relative expression of IL-6 in MIEN1-overexpressing PC3 cells treated with/without MK2206 as determined by RT-qPCR assays (± SE, n = 3). (G) The relative luciferase activity of IL-6 reporter vectors after transient overexpression of MIEN1 expression vector and treatment with/without MK2206 (± SE, n = 6). (H) Relative expression of IL-6 and MIEN1 after MIEN1 knockdown in PC3 cells determined by immunoblot and RT-qPCR assays. The number indicates the ratio of MIEN1/β-Actin in relation to PC-shCOL cells. (I) Relative luciferase activity of MIEN1 reporter vector after cotransfection with various dosages of IL-6 expression vector. (J) The relative luciferase activity of MIEN1 reporter vectors after transient overexpression of IL-6 expression vector and treatment with/without JSH-23 (± SE, n = 6). * p < 0.05, **,## p < 0.01.