Table 2.
Primers used for real-time quantitative PCR.
| Gene | Accession Number | Primer Sequence (5’–3’) | Size (bp) |
|---|---|---|---|
| β-actin | XM_003124280.5 | F: TGGAACGGTGAAGGTGACAGC R: GCTTTTGGGAAGGCAGGGACT |
177 |
| mTOR | XM_003127584.6 | F: GCACAAGGACGGATTCCTAC R: CACTTGCGTTGGGACATC |
248 |
| MuRF-1 | NM_001184756.1 | F: AACCTGGAGAAGCAGCTGAT R: TAGGGATTTGCAGCCTGGAA |
128 |
| Atrogin1 | NM_001044588.1 | F: TGGACTTCTCGACTGCCATT R: GCTATCAGTTCCAACAGCCG |
70 |
| HSL | NM_214315.3 | F: CACAAGGGCTGCTTCTACGG R: AAGCGGCCACTGGTGAAGAG |
167 |
| FASN | NM_001099930.1 | F: CTACGAGGCCATTGTGGACG R: AGCCTATCATGCTGTAGCCC |
146 |
| GCK | XM_013985832.2 | F: ATCAAACGGAGAGGGGACTT R: ACAATCATGCCAACCTCACA |
113 |
| MyHC I | NM_213855.1 | F: GTTTGCCAACTATGCTGGGG R: TGTGCAGAGCTGACACAGTC |
95 |
| MyHC IIa | NM214136.1 | F: CTCTGAGTTCAGCAGCCATGA R: GATGTCTTGGCATCAAAGGGC |
127 |
| MyHC IIb | NM_001123141.1 | F: GAGGTACATCTAGTGCCCTGC R: GCAGCCTCCCCAAAAATAGC |
83 |
| MyHC IIx | NM_001104951.2 | F: TTGACTGGGCTGCCATCAAT R: GCCTCAATGCGCTCCTTTTC |
111 |
mTOR = rapamycin target protein in mammalian cell; MuRF-1 = Muscle RING finger 1; Atrogin1 = Atrophy Gene 1; HSL = hormone-sensitive lipase; FASN = fatty acid synthase; GCK = glucokinase; MyHC I = heavy myosin-chain I; MyHC IIa = heavy myosin-chain IIa; MyHC IIb = heavy myosin-chain IIb; MyHC IIx = heavy myosin-chain IIx; F = forward; R = reverse.