Table A1.

Characteristics of the used cell lines and composition of the cell culture media used for each cell line.

Cell Lines	DU145	LNCaP	PC346C	PC346C-DCC	PC346C-Flu1
Culture Medium	RPMI 1640 FCS (10%) PS	RPMI 1640 FCS (10%) PS	DMEM/Ham’s F12 BSA (0.01%) FCS (2%) Epidermal growth factor (10 ng/mL) Insulin–transferrin–selenium (1%) Hydrocortisone (0.5 µg/mL) Triiodothyronine (1 nM) Phosphoethanolamine (0.1 mM) Cholera toxin (50 ng/mL) Fibronectin (100 ng/mL) Fetuine (20 µg/mL) R1881 (0.1 nM) PS	DMEM/Ham’s F12 BSA (0.01%) DCC (2%) Epidermal growth factor (10 ng/mL) Insulin–transferrin–selenium (1%) Hydrocortisone (0.5 µg/mL) Triiodothyronine (1 nM) Phosphoethanolamine (0.1 mM) Cholera toxin (50 ng/mL) Fibronectin (100 ng/mL) Fetuine (20 µg/mL) R1881 (0.1 nM) PS	PC346C-DCC medium plus OH-flutamide (1 µM)
AR	–	+	+	−	++
p53	Missense Mutation	Silent	WT	WT	WT
BRCA2	Missense Mutation	WT	WT	WT	WT
PTEN	WT	Frame shift deletion	Nonsense mutation	Nonsense mutation	Nonsense mutation

RPMI: Roswell Park Memorial Institute; DMEM: Dulbecco′s Modified Eagle′s Medium; BSA: Bovine serum albumin; AR: Androgen receptor; BRCA2: Breast cancer 2; PTEN: Phosphatase and tensin homolog; PS: Penicillin/streptomycin (100 U/mL, 100 µg/mL); FCS: Fetal calf serum; DCC: Charcoal dextran-stripped serum; WT: Wild type. For DU145 and LNCaP, data were acquired from The Cancer Cell Line Encyclopedia (CCLE) https://portals.broadinstitute.org/ccle.