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. 2019 Nov 6;19(3):161–173. doi: 10.3727/105221619X15529371970455

Figure 1.

Figure 1

Sterol 12α-hydroxylase (Cyp8b1) expression in diabetic and obese (db/db) mice. Wild-type C57BL/6J mice (n = 6) and db/db mice (n = 5) were sacrificed after 6 h of fasting. (A) Real-time PCR (qPCR) analysis of liver bile acid synthesis gene mRNA expression (left). Immunoblot of liver microsomal Cyp7a1, Cyp8b1, and Cyp7b1 protein expression in wild-type (n = 3) and db/db mouse liver (n = 5) (right). Calnexin was used as an internal control for microsomal proteins. (B) Mouse liver farnesoid X receptor (FXR) target gene mRNA expression in wild type and db/db mice. (C) Mouse intestine FXR target gene mRNA expression. Relative mRNA expression was calculated with respect to normal liver samples compared to human steatosis. Results are shown as means ± SE. Student’s t-test was used for statistical analysis. *Statistically significant difference (p < 0.05), WT versus db/db mice.