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. 2019 Nov 6;19(3):161–173. doi: 10.3727/105221619X15529371970455

Figure 6.

Figure 6

Effect of silencing Cyp8b1 in high-fat diet-induced obese (DIO) mice. Wild-type C57BL/6J mice were fed with HFD for 4 months. DIO mice were then injected with Ad-shCyp8b1 (n = 10) or Ad-shLacZ (Ad-shControl, n = 8) (indicated by an arrow) and were fasted for 6 h before sacrificed. (A) Body weight and glucose tolerance of Ad-shCYP8b1 DIO mice. Mice were fed HFD for 16 weeks. Ad-Cyp8b1 was injected to mice at time indicated by an arrow. Oral glucose tolerance test (left) and serum FGF21 levels (right). (B) Liver cholesterol and triglycerides. (C) Bile acid pool size. (D) qPCR analysis of liver Cyp8b1 and lipogenic gene mRNA expression. Immunoblot analysis shows that Ad-shCyp8b1 reduced Cyp8b1 protein levels in mouse livers (n = 3). (E) qPCR analysis of liver ceramide synthesis gene mRNA expression. (F) Immunoblot analysis of liver nSREBP-1c and phosphorylation of S6K. (G) qPCR analysis of liver inflammatory cytokine mRNA expression. (H) qPCR analysis of ileum FXR target gene mRNA expression. (I) Liver H&E staining of DIO + shControl and DIO + Cyp8b1 fed with high-fat diet for 4 months (representative of a liver each). Results are shown as means ± SE. Student’s t-test was used for statistical analysis. *Statistically significant difference (p < 0.05), Ad-shCyp8b1 versus Ad-shControl.