Figure 4.

SPARC-induced molecular changes. SW480 (A) and HCT116 (B) cells were seeded on collagen I. 750 ng/mL SPARC were added to the collagen gel or the culture medium. 24 h later protein lysates were prepared and analyzed by western blotting using antibodies recognizing integrin β1, FAK, p-FAK and E-cadherin. β–actin was used as loading control. Band intensity was measured using ImageJ software and quantification results of at least 3 independent cultures were pooled. (C) integrin β1, (D) relative phosphorylation of FAK, (E) E-cadherin in HCT116. * different from control at p < 0.05. FAK, focal adhesion kinase; p-FAK, phosphorylated FAK.