Table 2.
Summary of Data
Increased | PTH (μM) | PX (μM) | CPF (μM) | CPFO (μM) | DZN (μM) | DZO (μM) | DEDTP (μM) | DETP (μM) | DEP (μM) | |
---|---|---|---|---|---|---|---|---|---|---|
TNF-α | mRNA | 30, 100 | 30, 100 | 30, 100 | 0.001 and 0.1, 30, 100 | 30, 100 | 0.001 | 30 and 300 | ns | ns |
protein | 30, 100 | ns | 30, 100 | ns | 100 | ns | 300* | ns | ns | |
IL-1β | mRNA | 30, 100 | ns | 10, 30, 100 | 0.001 and 100 | 10, 30, 100 | 0.001 | 30 and 300 | ns | ns |
protein | ns | ns | ns | ns | 100 | ns | 300* | ns | ns | |
PDGF | mRNA | 10, 30, 100 | 0.001, 0.01, 0.1 | 1, 10, 30, 100 | 0.001, 0.01, 0.1 | 10, 30, 100 | 0.001 | 30 and 300 | ns | ns |
protein | ns | ns | ns | ns | ns | ns | — | — | — | |
TGF-β | mRNA | 10, 30, 100 | 100 | 30, 100 | 0.001, and 0.01, 100 | 30, 100 | ns | ns | ns | ns |
protein | nd | nd | nd | nd | nd | nd | — | — | — | |
NF-κB activation | 3, 10, 30, 100 | ns | 30 | ns | 10 | ns | — | — | — |
Definition of abbreviations: “and” = indicates no dose response effect; CPF = chlorpyrifos; CPFO = CPF oxon; DEDTP = O,O-diethyl dithiophosphate; DEP = diethyl phosphate; DETP = O,O-diethyl thiophosphate potassium salt; DZN = diazinon; DZO = diazoxon; nd = not detected (i.e., below the standard curve); ns = no significant increase; PTH = parathion; PX = paraoxon.
This table summarizes the data for PTH, PX, CPF, CPFO, DZN, and DZO, as well as DEDTP, DETP, and DEP on cytokine mRNA and protein release in THP1 cells. All concentrations are in μM. Data for PTH/oxon, CPF/oxon and DZN/oxon are from Figures 1 and E1. Protein levels for TGF-β were all less than the lower limit of the standard curve (lowest concentration 31.3 ng/ml). Data for NF-κB activation are from Figure 3. Data for DEDTP, DEP, and DETP are from Figures 2 and E3.
DEDTP at 300 μM caused a large increase in TNF-α and IL-1β, but it was not statistically significant.