Figure 5.

LT-e/w extract diminished the reactive oxygen species (ROS) accumulation significantly in SH-SY5Y cells under H₂O₂-induced oxidative stress. Cells were exposed to carboxy-H₂DCFDA for 15 min, following by 1 h (A) and 4 h (B) of incubation with diverse treatments. Subsequently, the ROS accumulation was measured via flow cytometer. Cells treated with DMSO or with 600 µM of H₂O₂ were included as controls. Each bar shows the mean of three biological replicates with its corresponding standard deviation. The significance of differences between 600 µM H₂O₂-treated cells, as compared to cells treated concomitantly with both LT-e/w extract plus 600 µM H₂O₂, were of p < 0.05 (*) or p < 0.01 (**), respectively.