Figure 7.

LT-e/w extract mitigated the H₂O₂-activated caspase-3 significantly in SH-SY5Y cells in a dose-dependent mode. Cells with active caspase-3 were stained with NucView 488 caspase-3 substrate and analyzed in a live-cell mode by flow cytometer. Each data point signifies the average of three biological replicates with its standard deviation. The asterisk(s) indicate a significant difference between cells treated concomitantly with both 15 µg/mL LT-e/w extract and 600 µM H₂O₂, as compared to cells treated with just 600 µM H₂O₂; p < 0.05 (*) and p < 0.01 (**), respectively. The Kaluza flow cytometry software (Beckman Coulter) was employed for acquisition and analysis purposes.