Figure 8.
LT-e/w extract attenuates the H2O2-induced PARP cleavage significantly in SH-SY5Y cells in a dose-dependent modality. Whole-cell lysates, with an equal amount of proteins in each lane, were subjected to western blot analysis for PARP cleavage determinations. (A) Representative western blot used for PARP cleavage analysis. (B) Each bar in the graph represents the average of densitometry analyses of two biological replicates with its corresponding standard deviation. (B) PARP cleaved band intensity values are relative to actin loading control protein. As a reference, lysates from solvent control (DMSO)-treated cells were included. Densitometry analysis was performed using the ImageStudio software suite (LI-COR).