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. 2019 Sep 23;11(10):1416. doi: 10.3390/cancers11101416

Figure 4.

Figure 4

TDP1 activity in GBM cell lines. (A) Gel assay for the conversion of radiolabeled P12Y DNA substrate into P12 product by recombinant human TDP1. The standard curve for the fraction of DNA substrate cleaved by increasing amounts of TDP1 is shown on the right; (B) TDP1 3’ endonuclease activity on hairpin substrate with 5’ fluorophore and 3’ quencher leads to increase in fluorescence. The initial velocity of fluorescence increase was used to generate a standard curve; (C) gel-based assay of relative TDP1 activity levels in GBM WCEs; (D) fluorescence-based assay of relative TDP1 activity levels in GBM WCEs. The bar graphs show the average and standard deviation as the error bar (n = 3 or more).