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. 2019 Sep 29;11(10):1466. doi: 10.3390/cancers11101466

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Differential miRNA methylation study in CMT samples. (A) Hierarchical clustering and heatmaps of DMRs on miRNA promoters were generated by complete linkage clustering using a Pearson correlation. Color scale represents percentage methylation. Hyper- (in red) and hypomethylation (in blue), N1, N2: normal, C1, C2: CMT. (B) Volcano plot presenting methylation differences in miRNAs consisting of known and new miRNA genes. Known miRNAs in green, new miRNAs in black; red indicates being chosen for validation, and gay is not significant. (C) Cfa-mir-96 and cfa-mir-149 expression levels determined by relative RT-qPCR in CMTs and adjacent normal tissues. The y-axis represents the log2 fold change value. (D) Dot plots of miRNA-targeted gene expression determined by RNA sequencing (RNA-seq) data in CMTs and adjacent normal tissues. Asterisks indicate * p < 0.05 and ** p < 0.01. miRNA: micro RNA; CMT: canine mammary tumor; DMR: differentially methylated region.