Figure 6.

Protein release from human endothelial cells. (a) Autoradiograph results demonstrating ⁵⁹Fe iron released by huECs is associated with transferrin. ⁵⁹Fe associated with Hft was undetectable. (b) Immunoblot demonstrating release of transferrin by huECs is comparable to ⁵⁹Fe-Tf standard. (c) Immunoblot demonstrating release of Hft by huECs comparable to ⁵⁹Fe-Hft standard. Lanes are as follows: (1) ⁵⁹Fe-Hft standard (Hft Std), (2) ⁵⁹Fe-Tf standard (Tf Std), (3) Control release experiment (Ctrl), (4) Release experiment with apo-Tf (+apo-Tf), (5) Release experiment with holo-Tf (+holo-Tf).