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. 2019 Oct 15;9(10):275. doi: 10.3390/brainsci9100275

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Neuroprotective activity and (B) reactive oxygen species (ROS) levels of NAR solution, NAR NPs, and placebo NPs were determined in SH-SY5Y cells using MTT assay on 6-OHDA induced neurotoxicity. Negative controls consisted of cells treated with 0.1% dimethylsulfoxide (DMSO) (vehicle control, VC) and cells treated with 6-OHDA alone without prior treatment with NAR solution and NAR NPs are positive control. Data was represented as % cell viability in a bar graph. Viability of control cells was 100%. Data are expressed as mean ± SD (n = 3). The data were analyzed using one-way ANOVA followed by Tukey’s post hoc test. Percentage of viable cells in each group was compared with VC, p < 0.05 and p< 0.01 indicates a significant and highly significant difference respectively.