Table 2.

List of primers used for amplicon sequencing protocol.

Segment	Primers name	Primer 5′-3′ sequence	Position*	Annealing temp	Size
#1	BVDV2 e2_2f	TGGCTGATGCTAATAACAGGGG	2433–2454	56 °C	1569
	BVDV2 NS3_2r	CTTGCTTGAACTCTCCTCTGTTCAT	3984–4006
#2	BVDV2 p7NS2_2f	GCTGACACACAATGATATTGAGGTTGTGGTC	3620–3650	60 °C	2184
	BVDV2 p7NS2_2r	GTTACATAGCTAGCTAGCAAAAG	5793–5817
#3	BVDV2 NS5f	AAGGAAGAACCTCAGCAGGGCCACCA	9321–9346	60 °C	1951
	BVDV2 NS5r	CTGTTATCAGAAAGCCATCATCACC	11259–11283

The positions (*) is based on the Bovine viral diarrhea virus 2 strain CN10.2015.821 sequence [MG879027].